spherical gold nanoparticles gnps  (BB International)

Journal: ACS Omega

Article Title: Tackling Anticancer Drug Resistance and Endosomal Escape in Aggressive Brain Tumors Using Bioelectronics

doi: 10.1021/acsomega.4c05794

Figure Lengend Snippet: Quantification of EVs released in drug-tolerant cell lines. (a–c) Cis-tolerant MB Group3 cells release significantly more EVs in comparison to their matched vehicle cell lines. For a detailed description of the cell lines and their identifiers used in this work, please refer to Section . Cis-resistant and cis-treated cell lines are denoted as DT-D458-Cis DT , DT-D283-Cis DT , DT-HD-MB03-Cis DT , and vehicle cell lines are denoted as DT-D458-DMF, DT-D283-DMF, and DT-HD-MB03-DMF line. (d) EVs release from vin-tolerant DAOY cells. Vin-resistant and vin-treated cell lines (DT-DAOY-Vin DT ) and vehicle (DT-DAOY-DMSO) cell lines. EVs in both cis- and vin-tolerant/vehicle cell lines were isolated using size-exclusion chromatography, and particle concentration from the pooled EVs-containing fractions was quantified using ZetaView nanoparticle tracking analysis. The concentration of EVs was calculated relative to 1 × 10 6 cells, where cells were counted at the point of harvest. Significant differences between vehicles and cis/vin-tolerant cells were calculated using an unpaired t test. The data represent the average ± SEM of three independent experiments. * p ≤ 0.05, ** p ≤ 0.01, and **** p ≤ 0.0001.

Article Snippet: The GIN and GCE cells were seeded in a 96-well plate at a density of 5 × 10 3 cells/well and allowed to adhere for 24 h. The medium was replaced with fresh medium containing Texas red conjugated 100 nm spherical gold nanoparticle (GNP, Nanopartz, Inc.) at different concentrations (25, 50, and 100 mg/mL), and the cells were incubated for 8 h. Cells with nanoparticle suspensions were then stimulated with AC of various frequencies at 1 V/cm for 30 min.

Techniques: Comparison, Isolation, Size-exclusion Chromatography, Concentration Assay